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. 2021 Feb 18;16(2):e0247132. doi: 10.1371/journal.pone.0247132

Fig 1. Esc2 and Mms21 have distinct and shared roles in maintaining cell growth and suppressing dGCRs.

Fig 1

A) Spot assays to measure hydroxyurea (HU) sensitivity (50 mM and 100 mM) of WT, esc2Δ, mms21-CH and mms21-CH esc2Δ mutants. The YPD plate is shown to show cell growth in the absence of drug. B) 5-FOA plasmid shuffling of a URA3 plasmid expressing ESC2 demonstrates the effect of evicting Esc2 in the siz1Δ siz2Δ esc2Δ triple mutant. C) dGCR rates of WT, esc2Δ, mms21-CH and esc2Δ mms21-CH mutants, measured via fluctuation analysis. D) dGCR fluctuation analysis demonstrating the effect of combining esc2Δ with rad52Δ, pol32Δ, rrm3Δ and rad9Δ. Error bars represent the upper and lower bounds of the 95% confidence interval with fold changes relative to WT shown above each respective analysis. For reference, mms21-CH specific data were taken from our previous studies.