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. 2021 Feb 18;16(2):e0247132. doi: 10.1371/journal.pone.0247132

Fig 3. Effect of esc2-D430R on cell growth and Ubc9 binding.

Fig 3

A) Sequence alignment of fungal Esc2 orthologs, highlighting the conserved D430 (ScEsc2) and E380 (SpRad60) residues. B) Western blot demonstrating protein expression levels of Esc2-D430R and WT Esc2. Endogenous C-terminal TAF tagged Esc2 proteins are detected by anti-Protein-A antibody, showing the D430R mutation does not appreciably affect the protein level of Esc2. C) Effect of the esc2-D430R mutation on Esc2 and Ubc9 binding. D) 5-FOA plasmid shuffling demonstrates the effect of esc2-D430R on the growth of the siz1Δ siz2Δ esc2Δ mutant, which is kept alive by a plasmid with WT ESC2. E) Representative tetrad dissection of diploids containing heterozygous mutations of siz1Δ, siz2Δ and esc2-D430R. F) Spot assays evaluating the growth properties of the siz1Δ, siz2Δ and esc2-D430R mutants.