Fig 5. SUMO proteomic approach to evaluate the effect of the esc2-D430R mutation on intracellular sumoylation via a comparison of the esc2-D430R mutant and WT cells.

Sumoylation levels of proteins in several functional groups are summarized here. A) Septins, B) NPC associated, C) Nucleolus and RNA Pol-I, D) the SMC complexes, E) DNA replication and repair and F) Chromosome segregation. Log₂ ratios of comparing the relative abundance of each protein in the WT vs the esc2-D430R mutant is shown, along with error bars representing the standard deviation calculated using multiple unique peptides of the same protein (see experimental method for details).