Table 1. Matrix effects in plant hormone determination.
| Tissue | Compound | Matrix effect (%) | S.D. |
|---|---|---|---|
| Leaf | IAA | 72.9 | 4.4 |
| cZ | 37.2 | 9.6 | |
| ABA | 53.8 | 3.7 | |
| GA4 | 51.1 | 3.7 | |
| SA | 54.5 | 14.7 | |
| JA | 50.9 | 15.8 | |
| BL | 67.9 | 4.7 | |
| Root | IAA | 46.2 | 4.0 |
| cZ | 10.2 | 4.6 | |
| ABA | 49.1 | 3.4 | |
| GA4 | 66.9 | 2.7 | |
| SA | 37.4 | 12.0 | |
| JA | 59.8 | 13.9 | |
| BL | 87.3 | 1.6 | |
| Stem | IAA | 62.9 | 9.4 |
| cZ | 33.5 | 6.6 | |
| ABA | 42.9 | 7.6 | |
| GA4 | 50.5 | 1.2 | |
| SA | 57.3 | 9.0 | |
| JA | 49.9 | 12.4 | |
| BL | 77.5 | 0.4 |
Hormones were extracted from the tissues of Lotus japonicus and mixed with seven pure standards. Ten ng/mL of phytohormone mixtures was analyzed with or without the matrix, and the peak areas were compared. Matrix effects are the mean values from three biological replicates.