Table 3. Quantification of plant hormones using standard addition.
| Tissue | Compound | Linear range (ng/ml) | Curve | R2 | LOD | LOQ | Content | RSD (%) | |
|---|---|---|---|---|---|---|---|---|---|
| ng/ml | ng/ml | pmol/g FW | SD | ||||||
| Leaf | IAA | 1.0–250.0 | Y = 77271*X − 141437 | 0.9995 | 0.1 | 0.5 | 95.1 | 4.0 | 4.3 |
| cZ | 10.0–250.0 | Y = 35908*X + 162509 | 0.9963 | 0.5 | 1.6 | N.D. | N.D. | N.D. | |
| ABA | 1.0–250.0 | Y = 66926*X − 29050 | 0.9998 | 0.1 | 0.3 | 185.7 | 84.4 | 45.4 | |
| GA4 | 1.0–250.0 | Y = 151474*X − 147592 | 0.9998 | 0.1 | 0.2 | N.D. | N.D. | N.D. | |
| SA | 1.0–250.0 | Y = 96955*X + 2118049 | 0.9917 | 0.2 | 0.7 | 2480.9 | 198.8 | 8.0 | |
| JA | 1.0–250.0 | Y = 69832*X − 1415498 | 0.9990 | 0.1 | 0.2 | 1023.8 | 94.2 | 9.2 | |
| BL | 1.0–250.0 | Y = 152721*X − 327260 | 0.9995 | 0.1 | 0.3 | 10.9 | 0.04 | 0.4 | |
| Root | IAA | 1.0–250.0 | Y = 140337*X − 13011 | 0.9993 | 0.1 | 0.3 | 71.8 | 10.3 | 14.3 |
| cZ | 10.0–250.0 | Y = 33924*X − 53632 | 0.9975 | 0.3 | 0.9 | N.D. | N.D. | N.D. | |
| ABA | 1.0–250.0 | Y = 92423*X − 229613 | 0.9995 | 0.1 | 0.3 | 38.8 | 2.8 | 7.1 | |
| GA4 | 1.0–250.0 | Y = 170129*X − 524708 | 0.9989 | 0.02 | 0.1 | N.D. | N.D. | N.D. | |
| SA | 1.0–250.0 | Y = 175984*X + 796822 | 0.9954 | 0.1 | 0.4 | 438.2 | 113.9 | 26.0 | |
| JA | 1.0–250.0 | Y = 74179*X + 1697965 | 0.9977 | 0.02 | 0.1 | 1129.7 | 347.4 | 30.7 | |
| BL | 1.0–250.0 | Y = 116960*X − 543073 | 0.9985 | 0.1 | 0.4 | N.D. | N.D. | N.D. | |
| Stem | IAA | 1.0–250.0 | Y = 103600*X − 263583 | 0.9987 | 0.1 | 0.2 | 239.6 | 11.0 | 4.6 |
| cZ | 10.0–250.0 | Y = 33302*X + 279101 | 0.9849 | 0.4 | 1.4 | N.D. | N.D. | N.D. | |
| ABA | 1.0–250.0 | Y = 78533*X − 134102 | 0.9986 | 0.1 | 0.4 | 161.4 | 20.2 | 12.5 | |
| GA4 | 1.0–250.0 | Y = 199797*X − 732980 | 0.9986 | 0.02 | 0.1 | N.D. | N.D. | N.D. | |
| SA | 1.0–250.0 | Y = 114085*X − 1541232 | 0.9866 | 0.5 | 1.6 | 1617.9 | 351.3 | 21.7 | |
| JA | 1.0–250.0 | Y = 57977*X + 2093537 | 0.9962 | 0.1 | 0.2 | 1430.1 | 462.6 | 32.3 | |
| BL | 1.0–250.0 | Y = 132286*X − 539659 | 0.9973 | 0.1 | 0.2 | N.D. | N.D. | N.D. | |
Seven phytohormones were extracted, and the calibration curves were constructed with sample matrix and analyzed by LC-MS/MS. Plant hormone extraction was performed in biological triplicate. R2, correlation coefficient; LOD, limit of detection; LOQ, limit of quantification; RSD, relative standard deviation; FW, fresh weight.