Fig 6. dMi-2 and Ush-B regulate genes associated with hemocyte functions.

A Volcano plot of deregulated genes upon depletion of dMi-2 in S2 cells. The -log₁₀(p-value) is plotted against the log₂ fold change of counts per gene in dMi-2-depleted (dsMi-2) vs. control cells (dsEGFP). Red dots represent significantly deregulated genes (adj. p < 0.01) obtained from biological triplicates (n = 3). B Gene ontology analysis of dMi-2-regulated genes. GO terms associated with hemocyte functions are highlighted in green. C Representative genes from all three gene classes regulated by Ush were analysed upon depletion of dMi-2 (dsMi-2) and Ush-B (dsUsh-B) by RT-qPCR. Gene names are indicated below. Expression was calculated relative to control treated cells (dsEGFP) and normalised using the mRNA levels of rp49. Error bars represent the standard deviation from biological replicates (n = 5) (T-test: *** p < 0.001, ** p < 0.01, * p < 0.05). Individual values of each replicate are displayed as circles.