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. 2021 Feb 18;17(2):e1009273. doi: 10.1371/journal.ppat.1009273

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© 2021 Le Sage et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — (A) Schematic of experimental procedure, gray bars depict 2-day exposure time. (B) All donor and recipient animals were infected with A/Perth/16/2009 (H3N2) virus 60 days prior to H1N1pdm09 (A/CA/07/2009) transmission. Donor animals are denoted as H3-H1 INF, and recipient animals are ‘H3-imm’. (C) All donor and recipient animals were infected with A/CA/07/2009 (H1N1pdm09) virus 84 days prior to H3N2 (Perth/16/2009) transmission. Donor animals are denoted as H1-H3 INF, and recipient animals are ‘H1-imm’. The contribution of donor and recipient immunity was tested separately for A/Perth/16/2009 (H3N2) transmission. Donors only (D) or recipient only (E) had pre-existing immunity to A/CA/07/09 H1N1pdm09. Donor animals are denoted as H1-H3 INF or H3N2 INF (no prior immunity), and recipient animals are either ‘naïve’ to indicate no prior immunity or ‘H1-imm’. Each bar represents an individual animal. Limit of detection is denoted by a dashed line.