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. 2021 Feb 18;17(2):e1009270. doi: 10.1371/journal.ppat.1009270

Fig 3. Expression levels of N. ceranae 16S rRNA.

Fig 3

The transcriptional activity of N. ceranae 16S rRNA was corresponding with the level of spore loads in infected bees. The relative expression of N. ceranae 16S rRNA in forager bees was significantly higher than in nurse bees under both high- and low- levels of infection (1.2 M vs 26 M N. ceranae spores per bee). The relative expression was expressed as an n-fold difference relative to the calibrator (marked by a star) by 2–∆∆Ct method. A star denotes a calibrator and an asterisk (*) denotes a statistically significant difference between the two groups (P ≤ 0.05, Student’s t-test).