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. 2021 Feb 18;17(2):e1009259. doi: 10.1371/journal.ppat.1009259

Fig 2. Red Blood Cell (RBC) surface expression of phosphatidylserine (PS) increases upon Plasmodium falciparum infection.

Fig 2

(A) Example images of Annexin V positive and negative RBCs, showing heterogeneity across the population. FITC-Annexin V fluorescence was detected at 475 nm (ex)/ 525 nm (em) and Hoechst fluorescence (parasite DNA) was detected at 390 nm (ex)/ 435 nm (em). Scale bar = 4 μm. (B) Mean percentage (± S.D.) of RBCs exposing PS in the membrane outer leaflet, measured by FITC-Annexin V staining above background levels (no Annexin V control) in flow cytometry, with and without 0.5 mM vanadate treatment to inhibit ATP hydrolysis. NS = not significant; ** = p < 0.01; *** = p < 0.001 (ANOVA). n = 3 independent experiments.