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. 2021 Feb 18;17(2):e1009259. doi: 10.1371/journal.ppat.1009259

Fig 6. Saponin-isolated parasites internalise NBD-lipids at the parasite plasma membrane.

Fig 6

(A) NBD-lipid internalisation, measured by increase in NBD mean fluorescence intensity (MFI) in flow cytometry after extraction of lipids remaining in the outer layer. Cells were treated with vanadate in calcium-free media to measure only the ATP-independent portion of internalisation. Shown are mean values (± S.D.), NS = not significant; *** = p < 0.001 (ANOVA). n = 3 independent experiments. (B) Subcellular localisation of NBD-PS (top), NBD-PE (centre), and NBD-PC (bottom) in saponin-isolated parasites from (A) after internalisation, visualised by deconvolution fluorescence microscopy. NBD-lipid fluorescence was detected at 475 nm (ex)/ 525 nm (em) and Hoechst fluorescence (parasite DNA) was detected at 390 nm (ex)/ 435 nm (em). Scale bar = 4 μm.