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. 2020 Dec 24;36(3):771–784. doi: 10.1093/humrep/deaa300

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© The Author(s) 2020. Published by Oxford University Press on behalf of European Society of Human Reproduction and Embryology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 8. — Spindle and chromosome alignments in in-vitro matured human oocytes in the presence of MitoQ. (A) Examples of aligned and misaligned chromosomes (arrows). Metaphase II spindles and chromosomes were visualized by staining tubulin and DNA to analyze chromosomal misalignment. (B) Percentage of mature oocytes with aligned and misaligned chromosomes in control and MitoQ groups. (C) Number of misaligned chromosomes per oocyte in control and MitoQ groups. (D) Comparison of spindle length in control and MitoQ groups. Error bars show SE of the mean. Number of oocytes analyzed in each group are shown. Chi-square test (B) or Student’s t-test (C and D) was used to compare between groups. *P < 0.05, NS, not significant.