Figure 1.

GYY4137 (100 μM) reduces CH cell numbers without inducing apoptosis. (A) Chemical structure of GYY4137. (B) CH metabolic activity was analyzed by performing an MTT following treatment with GYY4137 dose range (EC₅₀, 112.5±2.5 μM; n=4). (C) Quantification of CHs viability following treatment with different time with GYY4137 treatment. (D) Quantification of CHs viability following treatment with increasing concentrations of TBHP (E) Quantification of CHs viability following treatment with different time with 20 μM TBHP. (F) Quantification of CH viability following different dose of GYY4137 treatment following co-incubation with or without TBHP 20 μM. Data are presented as the means ± SEM (n=3). (C-F) ^*P<0.05 and ^**P<0.01 vs. control. CH, chondrocyte; TBHP, tert-Butyl hydroperoxide.