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. 2021 Feb 5;47(4):40. doi: 10.3892/ijmm.2021.4873

Figure 1.

Figure 1

CircUSP36 is upregulated in a model of AS in vitro. (A) HUVECs were exposed to various concentrations of ox-LDL (40, 60, 80 and 100 µg/ml) for 24 h, and cell viability was detected by CCK-8 assay (n=3). (B) HUVECs were exposed to 60 µg/ml ox-LDL and for different periods of time (6, 12, 24 and 48 h), and cell viability was detected by CCK-8 assay (n=3). In HUVECs exposed to 60 µg/ml ox-LDL for 24 h, (C and D) cell cycle distribution and cell apoptosis were monitored by flow cytometric assay (n=3). (E and F) Cell migration and invasion were assessed by Transwell assay (n=3). (G) The expression of IL-6 and IL-1β was detected by western blot analysis (n=3). (H and I) The release of IL-6 and IL-1β in medium was measured by ELISA (n=3). (J) The expression of circUSP36 in ox-LDL-treated HUVECs was measured by RT-qPCR (n=3). *P<0.05 vs. control. HUVECs, human umbilical vascular endothelial cells; ox-LDL, oxidized low-density lipoprotein.