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. 2021 Feb 5;47(4):40. doi: 10.3892/ijmm.2021.4873

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Copyright: © Miao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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ROCK2 is targeted by miR-20a-5p. (A) The potential association between miR-20a-5p and ROCK2 was predicted using the online tool, TargetScan. (B and C) The association between miR-20a-5p and ROCK2 was validated by dual-luciferase reporter assay and RIP assay (n=3). (D) The overexpression efficiency of miR-20a-5p was detected by RT-qPCR. (E and F) The effects of miR-20a-5p knockdown or overexpression on the expression of ROCK2 were examined by RT-qPCR and western blot analysis (n=3). (G and H) The expression of ROCK2 in ox-LDL-exposed HUVECs was measured by RT-qPCR and western blot analysis (n=3). ^*P<0.05 vs. respective control. HUVECs, human umbilical vascular endothelial cells; ox-LDL, oxidized low-density lipoprotein.