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. 2021 Feb 5;47(4):40. doi: 10.3892/ijmm.2021.4873

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Copyright: © Miao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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miR-20a-5p regulates ox-LDL-induced HUVEC injurys and inflammation by mediating ROCK2. (A and B) The inference efficiency of ROCK2 was examined by RT-qPCR and western blot analysis. HUVECs exposed to ox-LDL were transfected with si-ROCK2, si-NC, miR-20a-5p inhibitor + si-ROCK2 or miR-20a-5p inhibitor + si-NC. (C and D) Cell cycle distribution and cell apoptosis were monitored by flow cytometric assay (n=3). (E and F) Transwell assay was performed to observe cell migration and invasion (n=3). (G) The protein levels of IL-6 and IL-1β in cells were examined by western blot analysis (n=3). (H and I) The release of IL-6 and IL-1β in the culture medium was detected by ELISA (n=3). ^*P<0.05 vs. respective control. HUVECs, human umbilical vascular endothelial cells; ox-LDL, oxidized low-density lipoprotein.