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. 2021 Feb 2;47(4):35. doi: 10.3892/ijmm.2021.4868

Figure 4.

Figure 4

MIR181A2HG/miRNAs/AKT2 axis regulates proliferation, migration and capillary-like structures of HUVECs. (A-D) MTS assay, scratch test, Transwell migration, and 3D-culture were used to detect the cell viability, migration, and the formation of capillary-like structures. *P<0.05, **P<0.01 and ***P<0.001 vs. miR-Ctl. (E) Western blot analysis detected the expression of PCNA, MMP2 and VE-cadherin in HUVECs transfected with the different miRNAs. (F) Western blot analysis detected the expression of PCNA, MMP2 and VE-cadherin in HUVECs treated with the AKT2 inhibitor, MK2206. (G) MTS assay, (H) scratch test, (I) Transwell migration, and (J) 3D-culture were used to detect the cell viability, migration and the formation of capillary-like structures in HUVECs transfected with pGFP-N1-MIR181A2HG alone or together with shR-AKT2. **P<0.01 and ***P<0.001 vs. pGFP-N1+shR-Ctl; #P<0.05 and ##P<0.01 vs. pGFP-N1/MIR181A2HG+shR-Ctl. HUVECs, human umbilical vein endothelial cells.