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. 2021 Feb 2;47(4):35. doi: 10.3892/ijmm.2021.4868

Figure 5.

Figure 5

The promotion of proliferation and migration by MIR181A2HG is accompanied by changes of glucose metabolism. (A) ATP content, glucose uptake and glycogen synthesis were detected by luminescence assay, the glucose oxidase (GOD) method and glycogen colorimetric assay in HUVECs transfected with pGFP-N1-MIR181A2HG, shR-MIR181A2HG or their corresponding control. *P<0.05 and **P<0.01 vs. pGFP-N1, #P<0.05 vs. shR-Ctl. (B) ATP content, glucose uptake and glycogen were measured in HUVECs transfected with miR-6832-5p, miR-6842-5p and miR-8056. *P<0.05 and **P<0.01 vs. miR-Ctl. (C) ATP content, glucose uptake and glycogen were measured in HUVECs transfected with pGFP-N1-AKT2, shR-AKT2 and their corresponding control. *P<0.05 and **P<0.01 vs. pGFP-N1; #P<0.05 and ##P<0.01 vs. shR-Ctl. (D-F) Western blot analyiss was used to detect the expression of GLUT1, p-GSK3β and GSK3β in HUVECs treated as in (A-C). *P<0.05 and **P<0.01 vs. pGFP-N1 or miR-Ctl, #P<0.05 and ##P<0.01 vs. shR-Ctl. HUVECs, human umbilical vein endothelial cells.