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. 2021 Feb 2;47(4):35. doi: 10.3892/ijmm.2021.4868

Table I.

Sequences of primes used in the present study.

Primer name Sequence
AKT2-qPCR-Forward TGGTCGCCAACAGCCTCA
AKT2-qPCR-Reverse CC GCCACTTCCATCTCCTCA
MIR181A2HG-qPCR-Forward C GCGGTTCAATACCTCGTCT
MIR181A2HG-qPCR-Reverse TGCTGTGGCTAGAGGACAAC
β-actin-qPCR-Forward ACTCTTCCAGCCTTCCTTCC
β-actin-qPCR-Reverse C GTACAGGTCTTTGCGGATG
shRNA-MIR181A2HG-Forward GGGAUAGUAGAAAGUAACAGGCUCUTT
shRNA-MIR181A2HG-Reverse AGAGCCUGUUACUUUCUACUAUCCCTT
MIR181A2HG probe 1 human-392 CTTTCCACAGGACAGTTCGC
MIR181A2HG probe 2 human-377 TTCGCCTTTCCTTCCTTTCCTCTGT
MIR181A2HG probe 3 human-19 CCAGATTTCCTTGCAGCCC
miR-6832-5p mimics sense AGUAGAGAGGAAAAGUUAGGGUC
miR-6832-5p mimics antisense CCCUAACUUUUCCUCUCUACUUU
miR-6842-5p mimics sense UGGGGGUGGUCUCUAGCCAAGG
miR-6842-5p mimics antisense UUGGCUAGAGACCACCCCCAUU
miR-7110-5p mimics sense UGGGGGUGUGGGGAGAGAGAG
miR-7110-5p mimics antisense CUCUCUCCCCACACCCCCAUU
miR-6752-5p mimics sense GGGGGGUGUGGAGCCAGGGGGC
miR-6752-5p mimics antisense CCCCUGGCUCCACACCCCCCUU
miR-8056 mimics sense CGUGGAUUGUCUGGAUGCAU
miR-8056 mimics antisense GCAUCCAGACAAUCCACGUU
miR-181-3p mimics sense ACCACTGACCGTTGACTGTAC
miR-181-3p mimics antisense UACAGUCAACGGUCAGUGGUUU
miR-181-5p mimics sense AACATTCAACGCTGTCGGTGAGT
miR-181-5p mimics antisense UCACCGACAGCGUUGAAUGUUU
mimics Ctl sense UUCUCCGAACGUGUCACGUTT
mimics Ctl antisense ACGUGACACGUUCGGAGAATT
shRNA-AKT2-Forward C GUGGUGAAUACAUCAAGATT
shRNA-AKT2-Reverse UCUUGAUGUAUUCACCACGTT
miR-6832-5p-qPCR-Forward C GCTGCGAGTAGAGAGGAAAAG
miR-6832-5p-qPCR-Reverse TATGGTTCTTCACGACTGGTTCAC
miR7110-5p-qPCR-Forward C TTTATTATGGGGGTGTGGGG
miR-7110-5p-qPCR-Reverse TATGGTTGTTCACGAGTCCTTGTC
miR-8056-5p-qPCR-Forward CC TCGCTCGTGGATTGTCT
miR-8056-5p-qPCR-Reverse TATGGTTGTTCACCTCTCCTTCAC
miR-181-3p-qPCR-Forward C TTTATTAACCACTGACCGT
miR-181-3p-qPCR-Reverse TATGGTTCTTCACGACTGGTTCAC
miR-181-5p-qPCR-Forward C TTTATTAAACATTCAACGC
miR-181-5p-qPCR-Reverse TATGGTTCTTCACGACTGGTTCAC
U6-qPCR-Forward C GCTTCGGCAGCACATATAC
U6-qPCR-Reverse TTCACDAATTTGCGTGTCATC