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. 2021 Feb 1;47(4):30. doi: 10.3892/ijmm.2021.4863

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Copyright: © Bi et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effects of PSH on U251 cell apoptosis. (A) The rate of cell apoptosis was measured by Annexin V-FITC/PI staining and flow cytometry. (B and C) The activity levels of (B) caspase-3 and (C) caspase-9 were determined by ELISA. (D) Western blotting was used to determine the protein levels of cleaved caspases-3 and 9, Bax, cytochrome c and Bcl-2. The results are presented as the fold-change relative to the control group. Experiments were repeated three times, and the data are presented as the mean ± SD. ^*P<0.05 and ^**P<0.01 vs. control group. PSH, Paris saponin H.