Figure 3.

Effects of PSH on the U251 cell cycle. (A) U251 cells were treated with the indicated concentrations of PSH for 48 h, and the cell cycle distribution was determined by flow cytometry using PI staining. (B) The protein expression levels of P21, P27, cyclin D1 and Skp2 were measured by western blotting. The results are presented as the fold-change relative to the control group. Experiments were repeated three times, and the data are presented as the mean ± SD. ^*P<0.05 and ^**P<0.01 vs. control. PSH, Paris saponin H; Skp2, S-phase kinase associated protein 2.