Figure 6.

Effects of PSH on PI3K/Akt and 44/42 MAPK phosphorylation levels in U251 cells under hypoxia. (A) PSH inhibited the phosphorylation of PI3K/Akt and 44/42 MAPK. (B) U251 cells were co-treated with 100 µg/ml PSH and 20 µM LY294002 or U0126 for 48 h, and the protein expression levels of HIF-1α, VEGF, 44/42 MAPK and Akt were determined by western blotting. Experiments were repeated three times, and the data are presented as the mean ± SD. ^##P<0.01 vs. control; ^**P<0.01 vs. hypoxia; ^&&P<0.01 vs. PSH. PSH, Paris saponin H; MAPK, mitogen-activated protein kinase; HIF-1α, hypoxia-inducible factor-1α; VEGF, vascular endothelial growth factor; P-, phosphorylated.