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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Gastroenterology. 2020 May 17;159(3):984–998.e1. doi: 10.1053/j.gastro.2020.05.033

Figure 4. Influence of CRT overexpression on IECs intracellular creatine and barrier function.

Figure 4.

A. T84 IECs were transduced with lentiviral CRT ORF. CRT expression was assessed by qPCR(n=4). B. CRT protein was assessed by immunoblot in Vector and CRT OE cells(n=4). Densitometry was completed using ImageJ software. C. Intracellular CRT was assessed by creatine fluorometric assay with and without creatine supplementation to media. D. Cells were plated on a permeable membrane for transepithelial electrical resistance measurements (TEER)(n=3). E. FITC-dextran flux was measured using cells grown to maximum TEER(n=3). F. Wound healing was evaluated using the Incucyte wounder as previously described(n=4). G. Relative wound density was calculated incucyte ZOOM software. H. TJ protein expression was evaluated using qPCR of unstimulated cells(n=3). **p<0.01, ****p<0.0001 determined by one-way and two-way ANOVA analysis.