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. 2021 Jan 27;10:e63595. doi: 10.7554/eLife.63595

Figure 1. Chromatin accessibility landscape of facial chondrocytes.

(A) Confocal image of facial cartilages expressing col2a1a:GFP and sox10:Dsred at 72 hpf. Lateral view with anterior to left. Scale bar = 100 μm. (B) Venn diagram indicating distal elements with open chromatin accessibility in col2a1a:GFP+; sox10:Dsred+ versus col2a1a:GFP−; sox10:Dsred− cells. (C) Peak intensity plots (heatmap) of μATACseq show differentially enriched open chromatin regions in double-positive versus double-negative cells. (D) The top five transcription factor (TF) motifs recovered from the top 2000 μATACseq peaks enriched in chondrocytes (after removing redundant motifs). (E) GO analysis of nearest neighbor genes of μATACseq peaks enriched in chondrocytes.

Figure 1.

Figure 1—figure supplement 1. col2a1a enhancer and cartilage motif comparison between zebrafish and mouse.

Figure 1—figure supplement 1.

(A) col2a1a locus showing μATACseq reads from the indicated experiments. Several regions become accessible in 48 hpf CNCCs and then remain open in chondrocytes at 72 hpf. Boxed region shows the published R2 cartilage enhancer. (B) Comparison of consensus motif sequences recovered from zebrafish μATACseq peaks enriched in facial chondrocytes (left) and mouse Sox9 ChIP-Seq peaks in rib chondrocytes (right).