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. 2021 Jan 27;10:e63595. doi: 10.7554/eLife.63595

Figure 2. Dynamics of chromatin accessibility across facial chondrogenesis.

(A) Confocal images of CNCCs expressing fli1a:GFP and sox10:Dsred at 36 and 48 hpf. Lateral view with anterior to left. Scale bar = 100 μm. (B) Peak intensity plots of cartilage-accessible distal elements shown for chondrocytes at 72 hpf and CNCCs at 36 and 48 hpf. Chondrocyte accessible elements are pooled into three categories based on dynamics of chromatin accessibility across stages. (C, E, G) De novo motif enrichment recovered by Homer analysis among the three categories. Top six motifs are shown with associated p values after removing redundant motifs. (D, F, H) GO term analysis among the three categories.

Figure 2.

Figure 2—figure supplement 1. Comparison of accessible regions between 36 and 48 hpf CNCCs.

Figure 2—figure supplement 1.

(A) Peak intensity plots of μATACseq show differentially enriched open chromatin regions in 36 hpf and 48 hpf fli1a:GFP+; sox10:Dsred+ CNCCs. (B, C) De novo motif enrichment recovered by Homer analysis for peaks with decreasing (B) and increasing (C) accessibility from 36 to 48 hpf. Top six motifs are shown with associated p values after removing redundant motifs. (D, E) GO term analysis and associated p values for peaks with decreasing (D) and increasing (E) accessibility from 36 to 48 hpf.