Figure 6.

Karyopherin TNPO1 mediates nuclear import of CDK12. (a) PY-motif of the CDK12 of different species shows that the sequence of this domain is well conserved during evolution; central hydrophobic motifs and the R/K/H-PY motifs are highlighted in red. P: proline; Y: tyrosine. (b) Western blotting of TNPO1 levels following immunoprecipitation of CDK12 in HeLa and Siha cells. (c) After being transfected with siRNA targeting TNPO1 or inhibitor of the TRN pathway (M9M) vector, western blotting of CDK12 levels in the nucleoplasm and cytoplasm of HeLa and Siha cells. (d) HeLa and Siha cells were analyzed by immunofluorescence against CDK12, as labeled, during siTNPO1. DAPI staining was used to visualize nuclei. Scale bar: 20 μm.