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. 2021 Feb 19;296:100435. doi: 10.1016/j.jbc.2021.100435

Figure 2.

Figure 2

Sequence composition of RhACE2 cloned in this study with that of the wild-type RhACE2 and the susceptibility of RhACE2 proteins to SARS-CoV-2.A, two sites of natural variation (R192G and Y217N) were identified in the cDNA of RhACE2 cloned in this study, and wild-type RhACE2, mutant RhACE2, and hACE2 were compared. B, HEK 293T cells were transfected with plasmids expressing the indicated ACE2 protein. Cells were infected at a 0.5 MOI of SARS-CoV-2, and 24 h after transfection, IFA was used to detect the replication of SARS-CoV-2 in cells. The average entry ratio was analyzed by ImageJ software. C, HEK 293T cells were transfected with plasmids expressing the indicated ACE2 protein. Cells were lysed for western blotting 36 h after being transfected.