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. 2021 Jan 8;40(7):1255–1268. doi: 10.1038/s41388-020-01595-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A Transfection efficacy of two siRNAs for YAP1 (siYAP1 No.1 and No.2). B Cisplatin sensitivity of siYAP1-transfected ES-2 cells measured using MTS assay. The viability of treated cells was compared with that of cells transfected with negative control siRNA (siCtrl) using Student’s t test. C Apoptosis assay of transfected cells treated with 7.5 µM cisplatin. The percentage of apoptotic cells was compared using Student’s t test. D Cisplatin sensitivity of YAP1 inhibitor-treated ES-2 cells measured using MTS assay. The viability of treated cells was compared with that of cells treated with DMSO using Student’s t test. E Expression of miRNAs and YAP1 in YAP1 overexpressed ES-2 cells (FLAG-YAP1) and mock transfected cells (FLAG). The cells were further transfected with combination of miR-509-3p and miR-509-3-5p. F Cisplatin sensitivity of FLAG-YAP1 cells measured using MTS assay. The viability of the two miRNAs transfected cells was compared with that of NC transfected cells using Student’s t test. G Representative images and bar graph of clonogenic assay. The transfected cells were treated with 1 µM cisplatin for 8 days, and the number of colonies was compare using Student’s t test. H The expression of phospho-YAP (Ser127) in the cytoplasm and nucleus of FLAG-YAP1 cells. Error bars represent standard errors of the mean. *p < 0.05, **p < 0.01, and ***p < 0.001.