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. 2021 Jan 8;40(7):1231–1241. doi: 10.1038/s41388-020-01590-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Interaction between exogenous FLAG-MMSET and HA-CREB in HEK293 cells after transfection for 48 h immunoprecipitated using anti-FLAG antibody, and b reversely immunoprecipitated with anti-HA antibody. c Endogenous CREB and MMSET interaction in MM cells using MMSET antibody or d reversely using CREB antibody for immunoprecipitation. e Mapping of the interaction domain in MMSET required for CREB. f Interactions of HA-CREB and a series of FLAG-MMSET truncations (upper panel), and the lower panel shows expression profiles of FLAG-MMSET truncations. g Immunofluorescence staining of CREB and MMSET in MM.1S cells to show the subcellular co-localization. h Degradation of CREB in MM cells with MMSET knockdown under normoxia or hypoxia conditions. i Protective effects of the full length MMSET, or a SET domain depletion truncation, or a Y1179A mutant for loss of the methyltransferase function in the SET domain on CREB protein in HEK293T cells. j Degradation of CREB in MM cells infected with lentivirus-carrying vectors expressing full-length MMSET (FL), SET domain depletion truncation, or a Y1179A mutant for loss of the methyltransferase function in the SET domain for 72 h. Representative images are from at least n = 3 independent experiments for all Western blot and Co-IP assays.