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. 2020 Dec 12;101(3):353–368. doi: 10.1038/s41374-020-00512-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Transwell assay demonstrating the effects of CAF-like cells coculturing on the migration of TE-8, TE-9, and TE-15 cells. Migrating cells were counted in four randomly chosen fields. b Transwell assay demonstrating the effects of CAF-like cells coculturing on the invasion of TE-8, TE-9, and TE-15 cells. Invading cells were counted in four randomly chosen fields. c Transwell assay of the effects of PAI-1 neutralizing antibodies on the migration of TE-8, TE-9, and TE-15 cells cocultured with CAF-like cells. Normal goat IgG was the negative control for the PAI-1 antibody. d Transwell assay of the effects of PAI-1 neutralizing antibodies on the invasion of TE-8, TE-9, and TE-15 cells cocultured with CAF-like cells. Normal goat IgG was the negative control for the PAI-1 antibody. For (a–d), data represent the mean ± SEM of triplicate wells for three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001).