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. 2021 Feb 5;10:610545. doi: 10.3389/fonc.2020.610545

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Copyright © 2021 Jiang, Chen, Ge, Wang, Liu, Chen, Xu and Wu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overexpression of miR-21-5p induced cell death and repress cell viability in colorectal cancer (CRC) cells lines. (A) HT29 and HCT116 cells were transferred by miR-21-3p/miR-21-5p mimics and inhibitors, and cell death was determined by TUNEL stain assay. (B, C) Annexin V and PI staining flow cytometric analysis of cell death in miR-21-3p/miR-21-5p mimic and inhibitor transfected HT29 and HCT116 cells. (D) CCK-8 assay of HT29 and HCT116 cell viability changes with miR-21-3p/miR-21-5p mimics and inhibitors transfection. The bars and error bars indicate the mean ± SD. **p < 0.01, ****p < 0.001.