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. 2021 Feb 5;10:610545. doi: 10.3389/fonc.2020.610545

Figure 3.

Figure 3

Pathway evidence of miR-21-5p induced pyroptosis of colorectal cancer (CRC) cells. (A) ELISA assay tested the IL-1β and IL-18 which released in HT29 and HCT116 cell culture mediums by the transfection of miR-21-5p mimics and inhibitors. (B) Pyroptosis-associated mRNA levels in HT29 and HCT116 cells after miR-21-5p mimics and inhibitors transfection as determined by qRT-PCR. (C, D) Expression of Pyroptosis-associated proteins in HT29 and HCT116 cells after miR-21-5p mimics and inhibitors transfection as determined by western blot analysis. (E) Immunofluorescence staining of ASC specks expression in miR-21-5p mimics and inhibitors transfected HT29 and HCT116 cells. The bars and error bars indicate the mean ± SD. n.s.p > 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001.