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. 2021 Feb 5;11:609400. doi: 10.3389/fimmu.2020.609400

Figure 4.

Figure 4

Decreased expression of type II IFN signature genes in Ccr8-deficient mice with colitis. Ccr8+/+ and Ccr8−/− mice were subjected to the oral DSS treatment for 7 days. Total RNA of distal colonic specimens (day 9) was isolated and used for bulk RNAseq analysis. (A) Principal component analysis (PCA) of total variation in differentially expressed genes. (B) Hierarchical cluster analysis of differentially expressed genes among Ccr8+/+ and Ccr8−/− mice. (C) Volcano plot representation of gene expression changes. (D) Gene set enrichment analysis (GSEA) showing enrichment in IFN-γ regulated genes in Ccr8 +/+ mice compared to Ccr8 −/− mice. N = 3 mice/group. (E) The transcripts of selected genes in colonic tissue lysates were determined by specific qPCR. The data are represent one of three independent experiment (n = 3–5/group). (F) The concentration of IFN-γ and IL-22 in cell culture supernatants of LPMC stimulated for 48 h with PMA/Ionomycin was determined by ELISA. Pooled data from two independent experiments (n = 6/group). Statistical analysis was performed using Mann-Whitney U test. Results represent means ± S.E.M.: *P ≤ 0.05, ns, not significant.