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. 2021 Feb 5;11:609400. doi: 10.3389/fimmu.2020.609400

Figure 5.

Figure 5

CCL1/CCR8 signaling affects intestinal IFN-γ producing ILC1/ex-ILC3s. (A–E) LPMC cells were isolated from Ccr8 +/+ and Ccr8 −/− mice subjected to DSS-treatment (day 9), stimulated for 4 h with PMA/Ionomycin in the presence of a protein transport inhibitor and analyzed by flow cytometry. (F) Representative flow cytometric plots of CCR8 expression on ILC1s and NK cells using fluorophore-coupled CCL1 (CCL1-AF647) in Ccr8 +/+ and Ccr8 −/− mice. (G) LPMC cells were isolated from C57BL/6 mice overexpressing CCL1 or CCL8 subjected to DSS-treatment (day 9), stimulated for 4 h with PMA/Ionomycin in the presence of a protein transport inhibitor and analyzed by flow cytometry. Pooled data of two independent experiments with at least four mice in each group. Statistical analysis was performed using the Mann-Whitney U test or one-way ANOVA and the results were expressed in mean ± S.E.M.: *P ≤ 0.05; ***P ≤ 0.001, ns, not significant.