Figure 6.

CCL1/CCR8 axis protects from colitis in the absence of adaptive immune cells. Rag1 ^−/− mice were intravenously treated with 5 μg of CCL1 or control expression vectors. Two days later, the mice were exposed to 2% of DSS in drinking water for 7 days. (A) Relative body weight. (B) Representative H&E stainings of colonic cross sections and endoscopic pictures. Scale bar 100 µm. Histological and endoscopic scoring of mucosal damage. (C) Transcripts of IFN-γ and amphiregulin in colonic tissue lysates were determined by specific qPCR. (D) LPMC cells were isolated, stimulated for 4 h with PMA/Ionomycin in the presence of a protein transport inhibitor and analyzed by flow cytometry. The graphs show data of one representative experiment out of two independent experiments with four to six mice per group. Statistical analyses were performed using the Mann-Whitney U test. Data represent means ± S.E.M. n≥4 per group: *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001.