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. 2021 Feb 18;12(2):191. doi: 10.1038/s41419-021-03466-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A RT-qPCR showed the transfection efficiency of sh-MEG3 in HK-2 cells. B A caspase-3 kit was applied to detect the activity of caspase-3 in sh-MEG3 transfected HK-2 cells. C, D A TUNEL assay revealed the influence of MEG3 deficiency on apoptosis of HK-2 cells. E Western blot analysis indicated the protein levels of Parkin, p62, LC3B-I, and LC3B-II. F, G MitoTracker Green FM staining was used to evaluate mitophagy flux under the influence of MEG3 deficiency. Red color indicated DMSO and blue color indicated Baf. H The influence of MEG3 deficiency on ATP production was determined via an XFe96 extracellular flux analyzer. *p < 0.05, **p < 0.01.