Figure 1.

The ISW1b complex (Isw1-Ioc2-Ioc4) is required for setting wild type nucleosome spacing, whereas the ISW1a complex (Isw1-Ioc3) plays only a minor role. Average nucleosome dyad density plots for all genes: (a) wild type, (b) isw1∆, (c) ioc3∆, (d) ioc2∆, (e) ioc4∆, (f) ioc2∆ ioc4∆, (g) ioc2∆ ioc3∆ ioc4∆. All yeast genes were aligned on the midpoints of their + 1 nucleosomes. The dyad distribution was normalized to the global average (set at 1). For ease of comparison, wild type (WT) replicate A is shown as a black line with grey fill in all plots. Two biological replicate experiments (A and B) are shown for each strain in all of the plots (A: red line; B: blue line). The average spacing in bp is shown for replicates A and B in the bottom right corner (measured by regression analysis of the first 5 nucleosome peaks, beginning with the + 1 nucleosome).