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. 2021 Feb 18;11:4083. doi: 10.1038/s41598-021-81697-4

Figure 2.

Figure 2

Syntenin-knock out reduces the exosomal secretions of syntenin cargo by primary MEFs in culture. (a) Exosome characterization by nanoparticle-tracking. Exosomes were collected from equivalent amounts of culture medium, conditioned by equal numbers of cells, for equal lengths of time. Particle numbers and sizes were analyzed by Nanosight. (b) Left: Western blot analysis of proteins present in the lysates and exosome preparations of WT and Synt KO MEFs in primary culture. Exosomes were collected from equivalent amounts of culture medium, conditioned by equal numbers of cells, for equal lengths of time. Right: Bar graphs showing the levels of exosomal marker proteins in Synt-KO MEFs, relative to the levels measured in WT cells (taken as 100%). Note the concurrent decreases of ALIX, CD63 and syndecan 1-CTF (SDC1-CTF), and potential syndecan cargo such as B1-integrin, fibronectin, and EGFR, but not CD81 and TSG101, in the exosomes of Synt KO MEFs.