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. 2021 Feb 5;7:571999. doi: 10.3389/fvets.2020.571999

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Copyright © 2021 Kalaiyarasu, Bhatia, Mishra, Senthil Kumar, Kumar, Sood, Rajukumar, Ponnusamy, Desai and Singh.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Lymphocyte proliferation assay of M2e- and M2e–HA2-immunized and non-immunized control chickens. Stimulation index (SI) was increased gradually from day 0 to day 14 in the M2e and M2e–HA2 groups. SI by lipopolysaccharide (LPS) with all the groups was maximum and higher than concanavalin A (ConA) from day 14 and reached the peak at day 21 DPV. Data are means ± SD (n = 6), statistical significance (*p ≤ 0.05, **0.01) vs. non-immunized control groups in the same time interval. The control mentioned within group is the unstimulated lymphocytes of the same group.