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. 2021 Feb 5;7:571999. doi: 10.3389/fvets.2020.571999

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Copyright © 2021 Kalaiyarasu, Bhatia, Mishra, Senthil Kumar, Kumar, Sood, Rajukumar, Ponnusamy, Desai and Singh.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of relative expression of Toll-like receptor (TLR) mRNAs in M2e- and M2e–HA2-immunized and non-immunized control chicken peripheral blood mononuclear cells (PBMCs). M2e–HA2-immunized chickens showed upregulation of all the TLR mRNAs than M2e and control on 3 and 7 days post-immunization. Data are means ± SD (n = 6), statistical significance (*p ≤ 0.05, **0.01) vs. control non-immunized groups in the same time interval.