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Comparison of relative expression of inflammatory cytokines mRNA in M2e, M2e–HA2, and control chicken peripheral blood mononuclear cells (PBMCs) followed by challenge with clade 2.3.2.1 H5N1. M2e–HA2-immunized chickens showed drastic reduction of interleukin (IL)-1β and IL-6 than M2e and control. Data are means ± SD (n = 6), statistical significance (*p ≤ 0.05, **0.01) vs. control-infected groups in the same time interval.
