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. 2021 Feb 5;10:593293. doi: 10.3389/fonc.2020.593293

Figure 1.

Figure 1

DDX11 expression is increased in HCC tissues. (A) The mRNA expression of DDX11 was determined in 24 pairs of fresh HCC and nontumorous (NT) samples, using qRT-PCR in SYSUCC cohort (Matched pair t test). (B) The increased expression of DDX11 mRNA was validated in 50 paired HCC tissues in TCGA cohort (Matched pair t test). (C) GEO studies indicate the level of DDX11 mRNA in HCC cases was higher than that in nontumorous (NT). (D) Proteins extracted in clinical samples were subjected to western blot to examine the DDX11 protein expression. β-actin was used as a loading control. T, tumor samples; N, nontumorous samples. (E) A large cohort containing 328 HCC cases was recruited. The protein level of DDX11 was measured by IHC in paraffin-embedded tissues. Representative images and relative IHC scores were presented in the left and right panels, respectively. (F) Another cohort consisting of 47 paired primary HCC and corresponding portal vein tumor thrombus tissues was collected to detect the expression of DDX11 by IHC.