Figure 3.

DDX11 promotes cell proliferation in HCC. (A) Cells were transfected with DDX11 shRNA and selected by G418 to construct stable cell lines with DDX11 knockdown. qRT-PCR and western blot were used to confirmed the downregulation of DDX11 mRNA and protein in HepG2 and PLC8024 cells. *P < 0.05. (B) The effect of DDX11 silence on HCC cell growth were determined by MTT assays. Stable cells were placed into 96-well plates to continue to grow for 4 days. Cell growth rates were calculated by the absorbance at OD490 nm. *P < 0.05, **P < 0.01. C. Colony formation was performed to validate the role of DDX11 on cell proliferation. Stable cells were cultured in 60 mm plates with G418-contained DMEM for 10 days. Colonies were pictured and counted under a microscope (left panel). The fold change (FC) of colony formation was indicated by histogram (right panel). *P < 0.05. (D) Transwell assays were performed to assess whether DDX11 affects cell migration. Cells transferred to the bottom side of the Transwell chamber were stained by 0.1% crystal violet and counted. The fold change (FC) of cell migration were shown. *P < 0.05. (E–H) Stable cells with DDX11 overexpression were constructed (E) and used in the determination of DDX11-mediated cell growth by MTT (F), colony formation (G) and Transwell (H) assays. *P < 0.05, **P < 0.01.