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. 2021 Feb 18;11:4060. doi: 10.1038/s41598-021-83606-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Chemosensitizing effect of L. squarrosulus peptide in multicellular lung cancer spheroids. PrestoBlue assay presented the significant reduction of %cell viability in cancer spheroids obtained from lung cancer (a) H460, (c) H23, (e) H292 and (g) A549 cells that were cultured with 0.4 M NaCl peptide (5 µg/mL) for 24 h prior to 24 h treatment of 5 µM cisplatin. Nuclear staining clearly depicted the elevation of apoptosis observed as bright blue fluorescence of Hoechst33342 in multicellular spheroids of (b) H460, (d) H23, (f) H292 and (h) A549 cells after coculture with L. squarrosulus peptide and cisplatin. Notably, the presentation of both bright blue Hoechst33342 fluorescence and red fluorescence of propidium iodide (PI) indicated late stage of apoptosis detected in the spheroids after preincubation with 0.4 M NaCl peptide followed by cisplatin treatment. Values are means of three independent experiments ± SD. *p < 0.05 versus non-treated control cells, ^#p < 0.05 versus the cells treated only with cisplatin.