Table 5.

Strengthening of diagnostics for epidemic prevention and containment using PANDAA.

PANDAA design feature	Benefit
PANDAA can adapt de novo probe-binding site sequence variants.	An a priori understanding of all probe-binding site variations does not need to be known. Impact of position and nucleotide substitution type on sensitivity can be determined in advance.
PANDAA is a lineage- / strain-agnostic assay by virtue of the adaptation process.	Global diagnostic harmonization without the need for individual assays to address geographical genomic variability.
PANDAA’s inherent tolerance of extreme primer degeneracy favors the development of multiplexed assays.	Detect and differentiate multiple subtypes in a single reaction e.g., differentiation of circulating influenza virus from pandemic strains25,45; reduced need to update diagnostics based on seasonal genetic drift.