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. 2021 Feb 18;11:4115. doi: 10.1038/s41598-021-83645-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Endometriosis modelling in vitro. Left. Sampson’s theory of retrograde menstruation. Menstrual tissue contains stromal condensates (dark blue) and collapsed epithelial glands (pink). Ectopic lesions are frequently described to have a ‘bullet-like appearance’ Right. Spheroids generated using the hanging drop method as a model of collapsed endometrium architecture are placed on either Matrigel or collagen I on day 4 and their phenotype on the hydrogels and the effect of pharmacological intervention is evaluated.