Fig. 5. Morphological phenotyping based on automated quantitative measurements.

a Gaussian fitting curves of the angle distribution statistics of 15 larvae in the control group. The averaged variances of the angle distribution for edema and tail evaluation are ${\sigma }_{0E}^2=0.0237\pm 0.0091$ and ${\sigma }_{0T}^2=0.0085\pm 0.0032$, respectively. b Statistical distribution of the “abnormality factors” ($R={\sigma }^2/{\sigma }_0^2$) for edema and tail curling in the control group (n = 15) and the 1.5%-EtOH group (n = 35). Based on statistical analysis, the larvae within the range of R ≤ 1.5, 1.5 < R ≤ 4, and R > 4 are categorized as unaffected, moderate, and severe, respectively, for morphological abnormality level. c The percentage of phenotype classification (as unaffected, moderate, or severe) of the morphological abnormalities after the 1.5% EtOH exposure. Source data is available as a source data file.