Figure 3.

Unique acylcholines profile in EC

(A) Network analyses of the metabolites that were significantly different in EC versus HC (154 metabolites). Rectangular shapes represent the eight super-pathways that are shown in different colors according to legend. Octagonal shapes are used for subpathways belonging to the eight super pathways. Circular shapes show the single metabolites, where red indicates increased levels and green indicates decreased levels in EC relative to HC. The size of the circles indicates p value: the bigger the size, the lower the p value. Lines connect each metabolite to its respective subpathway and subpathway to their respective super-pathways. See also Figure S4 for network analyses between EC and VP as well as HC and VP.

(B–E) (B–D) Network showing the sub pathway Fatty acid metabolism acylcholines and metabolites belonging to it, with red indicating increased levels and green indicating decreased levels. Size of the circles indicated p value of (B) EC versus HC, (C) EC versus VP, and (D) VP versus HC. (E) Spearman correlation analyses between metabolites were performed for EC samples and revealed a moderate correlation between levels of cysteine-glutathione disulfide and palmitoylcholine (R = 0.6, p value = 0.032) as well as levels of cysteine-glutathione disulfide and docosahexaenoylcholine (R = 0.59, p = 0.036). Respective scatterplots are presented. Data shown include samples of HC (n = 12), EC (n = 13), and VP (n = 16).