Figure 3.

VLCFA β-oxidation activities and cellular VLCFA levels in the immortalized astrocytes. The total cellular proteins (150 μg protein per lane) that were prepared from the primary astrocytes (WT and KO) as well as the immortalized astrocytes (WT5 and KO9) were analyzed by immunoblotting using an anti-Abcd1, anti-Abcd2, anti-Abcd3 and anti-actin primary antibody, respectively (A). The transferred proteins were checked by staining with Ponceau S. Full images were represented in supplementary materials (Suppl. Fig. 2). VLCFA β-oxidation activities in the immortalized astrocytes (WT5 or KO9) were measured using [1-¹⁴C]C24:0 as the substrate (B). The VLCFA β-oxidation activities of WT5 (gray bars) and KO9 (black bar) are expressed as nmol/mg/h. The results are presented as the means ± S.D.; n = 4. (∗, p < 0.02 vs. the WT5). Total fatty acids were extracted from immortalized astrocytes (WT5 or KO9) and analyzed by gas-liquid chromatography (C). The values of WT5 (gray bars) and KO9 (black bar) are expressed as C26:0 ng/mg protein. The data are the mean ± S.D. (∗, p < 0.02 vs. the WT).