Fig. 3. Analysis size and shape of N-TMCNS loaded with tetanus toxoid with different methods. (A) Scanning electron microscope image of TT-loaded N-TMCNS, (B) AFM, and (C) hydrodynamic particle size distribution images of N-TMCNS containing TT. (A) The average diameter of the produced TT loaded N-TMCNS, which are monodispersed spherical nanoparticles, was found to be about 90 nm. (B) AFM result showed that nanospheres was about 90 nm in the dynamic light scattering method, and (C) two types of nanospheres with a diameter of 40 and 150 nm were observed. N-TMCNS, N-trimethyl chitosan nanospheres; TT, tetanus toxoid; AFM, atomic force microscope.