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. 2021 Feb 5;9:633331. doi: 10.3389/fcell.2021.633331

Figure 3.

Figure 3

5μM Rapamycin treatment of UVA-induced photoaged HDFs promoted increased autophagy, reduced the expression of p-HSP27 and p53, and decreased the stress levels of cells. (A,B) Western blots of p62, LC3 B, p-HSP27, and p53 expression after 24 h of rapamycin treatment. Proteins were quantified using β-actin as reference and expressed as a fold change in protein levels compared to control cells. Results are expressed as mean ± SD of at least three experiments (*p < 0.05, **p < 0.01, ***p < 0.005). (C–F) RT-PCR for mRNA expression levels of p62, LC3 B, p-HSP27 and p53. Results are expressed as mean ± SD of at least three assays (*p < 0.05, **p < 0.01, ***p < 0.005).